Journal: 3 Biotech

Article Title: The alpha-amylase MrAMY1 is better than MrAMY2 in rice starch degradation, which promotes Monascus pigments production in Monascus ruber

doi: 10.1007/s13205-019-2026-8

Figure Lengend Snippet: The 13 predicted alpha-amylases of M.ruber NRRL1597 from the KOG functional classification

Article Snippet: In addition, they were all part of the glycoside hydrolase family 13, but belonged to different sub-families, such as subf1(P440333, P324551), subf5(P472279), subf40(P379161, P411620, {"type":"entrez-protein","attrs":{"text":"P63242","term_id":"54037410","term_text":"P63242"}} P63242 , P454978, P460054, P501041), subf22(P464710, P469192, P469571), and subf25(P435885). table ft1 table-wrap mode="anchored" t5 Table 1 caption a7 Protein ID EC number Best hit CAZy database Length of gene (bp) Number of exons 440333 EC:3.2.1.1 gi|212,541,000|, alpha-amylase, putative, Penicillium marneffei ATCC 18,224 Identity: 65% GH13_1 a 1706 2 324551 EC:3.2.1.1 gi|350,636,595|, alpha-amylase, Aspergillus niger ATCC 1015 Identity: 57% GH13_1 2145 8 472279 EC:3.2.1.1 gi|317,140,381|, alpha-amylase, Aspergillus oryzae RIB40 Identity: 68% GH13_5 2125 8 379161 EC:3.2.1.20 aor:AO090026000034, alpha-amylase, Aspergillus oryzae Identity: 72% GH13_40 1860 3 411620 EC:3.2.1.20 afm:Afu3g07380,oligo-1,6-glucosidase, Aspergillus fumigatus Af293 Identity:79% GH13_40 1834 2 63242 EC:3.2.1.20 gi|242,806,039|, maltase, Talaromyces stipitatus ATCC 10,500 Identity: 65% GH13_40 1907 1 454978 EC:3.2.1.20 afm:Afu2g11620, oligo-1,6-glucosidase, Aspergillus fumigatus Af293 Identity: 79% GH13_40 2389 10 460054 EC:3.2.1.20 gi|345,564,184|, hypothetical protein AOL_s00097g3, Arthrobotrys oligospora ATCC 24,927 Identity: 48% GH13_40 1934 3 501041 EC:3.2.1.20 gi|119,481,197|, alpha-glucosidase/alpha-amylase, putative, Neosartorya fischeri NRRL 181 Identity: 76% GH13_40 2295 9 464710 EC:2.4.1.183 gi|242,818,376, alpha-1,3-glucan synthase, putative, Talaromyces stipitatus ATCC 10,500 Identity: 57% GH13_22 7859 11 469192 EC:2.4.1.183 afm:Afu2g11270, alpha-1,3-glucan synthase, Aspergillus fumigatus Af293 Identity: 71% GH13_22 7595 6 469571 EC:2.4.1.183 aor:AO090010000106, glycogen synthase, Aspergillus oryzae Identity: 80% GH13_22 7428 4 435885 EC:3.2.1.33 gi|119,497,357|, amylo-alpha-1,6-glucosidase, putative, Neosartoryafischeri NRRL 181 Identity:73% GH13_25 4761 3 Open in a separate window a The GH13_1 represented Glycoside Hydrolase Family 13 / Sub-family 1 The 13 predicted alpha-amylases of M.ruber NRRL1597 from the KOG functional classification

Techniques: Functional Assay

Journal: Journal of microbiological methods

Article Title: Novel Disk diffusion Assay for Filamentous Fungi Susceptibility to antifungals (DAFFS).

doi: 10.1016/j.mimet.2025.107145

Figure Lengend Snippet: Fig. 2. Application of the DAFFS method for other antifungal drugs and filamentous fungi genera. Schematic of disk dose concentration placement created using BioRender (Urbin, 2024) (A). Example plates of T. marneffei and Caspofungin treatment after 11 days growth (B), L. ramosa and Amphotericin B treatment after 2 days of growth (C), and T. asperellum and Amphotericin B treatment after 5 days of growth (D).

Article Snippet: The strains used in these studies, Trichoderma asperellum (environmental isolate), Talaromyces marneffei (ATCC 18224D-2), and Lichtheimia ramosa (ATCC 22754) were grown on RPMI 1640 agar plates (see Section 2.2) without antifungals to verify compatibility of growth medium and characterize growth rate on RPMI 1640 prior to experimentation with the DAFFS method.

Techniques: Concentration Assay

Journal: Viruses

Article Title: Spatial and Temporal Pattern of Norovirus Dispersal in an Oyster Growing Region in the Northeast Pacific

doi: 10.3390/v14040762

Figure Lengend Snippet: Primers and probes sequences, and source for RTqPCR assays used in this study. Probes were labelled with 5′-carboxyfluorescein (FAM) and 3′-black hole quencher-1 (BHQ-1).

Article Snippet: Oysters were tested for GI and GII HuNoV according to the ISO 15216-1_2017-03 method “microbiology of food and animal feed—horizontal method for determination of HAV and NoV in food using real-time PCR” with the exception that bacteriophage MS2 (ATCC ® 15597-B1 TM ) was used as the process control virus.

Techniques: